For an updated list of publications press here
Selected publications
Liu^, J., Shah^, A., Ma, Y., Hardman, K., Johansson, N.J., Ribeiro, O., Brookfield, A., Bowen, A., Yli-Kauhaluoma, Y., Xhaard, H., Jeuken, L.J.C., Goldman*, A., Pliotas*, C., Vidilaseris*, K. (2024) Conformational dynamics and asymmetry in multimodal inhibition of membrane-bound pyrophosphatases. eLife (doi: 10.7554/eLife.102288.1)
Lane^, B.J., Ma^, Y., Yan^, N., Wang, B., Ackermann, K., Karamanos, T.K., Bode, B.E., and Pliotas*, C. (2024) Monitoring the Conformational Ensemble and Lipid Environment of a Mechanosensitive Channel Under Cyclodextrin-Induced Membrane Tension. Structure 32: 1-12 (doi: 10.1016/j.str.2024.02.020)
Haysom, S.F., Machin, J., Whitehouse, J.M., Horne, J.E., Fenn, K., Ma, Y., Hassane El Mkami, Nils Böhringer, Schäberle, T.F., Ranson, N.A., Radford*, S.E., and Pliotas*, C. (2023) Darobactin B Stabilises a Lateral-Closed Conformation of the BAM Complex in E. coli Cells. Angew Chem Int Ed p.e202218783 (doi: 10.1002/ange.202218783) – Selected inside Cover in Angewandte Chemie International Edition
Wang^ B., Lane^, B. J., Kapsalis, C., Ault, J. R., Sobott, F., El Mkami, H., Calabrese, A. N., Kalli, A. C., Pliotas*, C. (2022) Pocket delipidation induced by membrane tension or modification leads to a structurally analogous mechanosensitive channel state. Structure 30: 1-15 (doi: 10.1016/j.str.2021.12.004)
Kapsalis, C., Wang, B., El Mkami, H., Pitt, S. J., Schnell, J. R., Smith, T. K., Lippiat, J. D., Bode, B. E., Pliotas*, C. (2019) Allosteric activation of an ion channel triggered by modification of mechanosensitive nanopockets. Nat Commun 10:4619. (doi: 10.1038_s41467-019-12591-x)
Pliotas, C., Dahl, A. C. E., Rasmussen, T., Mahendran, K. R., Smith, T. K., Marius, P., Gault, J., Banda, T., Rasmussen, A., Miller, S., Robinson, C. V., Bayley, H., Sansom, M. S. P., Booth, I. R., and Naismith*, J. H. (2015) The role of lipids in mechanosensation. Nat Struct Mol Biol (doi: 10.1038/nsmb.3120)
All publications
2025
30. Lane, B.J., Dionysopoulou, M., Yan, N., Lippiat, J.D., Muench, S.P., Pliotas*, C. (2025) The mechanosensitive channel YbiO has a conductance equivalent to the largest gated-pore. Structure (accepted)
29. Johnson, A.V., Porav, S.A., Hammond, K.L.R., Shah, A., Wort, J.L., Ma, Y., Mkami H.El,, Pask, C.M., Muench, S.P., Wilson, A.J., Pliotas*, C., Bon*, R.S. (2025) Non-covalent spin labelling enables EPR studies of structure and ligand interactions of a human TRP channel in solution. ChemRxiv (doi: 10.26434/chemrxiv-2024-t3bp9)
28. Shah^, A., Wort^, J.L., Ma, Y., Pliotas*, C. (2025) Enabling structural biological EPR spectroscopy in membrane proteins through spin labelling. Curr Opin Chem Biol (accepted)
2024
27. Liu^, J., Shah^, A., Ma, Y., Hardman, K., Johansson, N.J., Ribeiro, O., Brookfield, A., Bowen, A., Yli-Kauhaluoma, Y., Xhaard, H., Jeuken, L.J.C., Goldman*, A., Pliotas*, C., Vidilaseris*, K. (2024) Conformational dynamics and asymmetry in multimodal inhibition of membrane-bound pyrophosphatases. eLife (doi: 10.7554/eLife.102288.1)
26. Lane^, B.J., Ma^, Y., Yan^, N., Wang, B., Ackermann, K., Karamanos, T.K., Bode, B.E., and Pliotas*, C. (2024) Monitoring the Conformational Ensemble and Lipid Environment of a Mechanosensitive Channel Under Cyclodextrin-Induced Membrane Tension. Structure 32: 1-12 (doi:10.1016/j.str.2024.02.020)
2023
25. Hardman, K., Goldman, A., and Pliotas*, C. (2023) Membrane force reception: mechanosensation in G-Protein Coupled Receptors and tools to address it. Curr Opin Physiol 100689 (doi: 10.1016/j.cophys.2023.100689)
24. Haysom, S.F., Machin, J., Whitehouse, J.M., Horne, J.E., Fenn, K., Ma, Y., Hassane El Mkami, Nils Böhringer, Schäberle, T.F., Ranson, N.A., Radford*, S.E., and Pliotas*, C. (2023) Darobactin B Stabilises a Lateral-Closed Conformation of the BAM Complex in E. coli Cells. Angew Chem Int Ed, p.e202218783 (doi: 10.1002/ange.202218783)
23. Lane, B. J., and Pliotas*, C. (2023) Approaches for the modulation of mechanosensitive MscL channel pores. Front Chem 11:1162412 (doi.org/10.3389/fchem.2023.1162412)
2022
22. Boakes, J. C., Harborne, S. P. D., Ngo, J. T. S., Pliotas, C., Goldman*, A. (2022) Novel variants provide differential stabilisation of human equilibrative nucleoside transporter 1 states. Front Mol Biosci 9: 970391 (doi.org/10.3389/fmolb.2022.970391) (Editor selection for the special collection “Understanding Membrane Transporters: From Structure to Function”)
21. Dionysopoulou, M., Yan, N., Wang, B., Pliotas*, C., Diallinas*, G. (2022) Genetic and cellular characterization of MscS-like putative channels in the filamentous fungus Aspergillus nidulans. Channels 16: 148-158 (https://doi.org/10.1080/19336950.2022.2098661)
20. Lane, B. J., Wang, B., Ma, Y., Calabrese, A. N., El Mkami, H., Pliotas*, C. (2022) HDX-guided EPR spectroscopy to interrogate membrane protein dynamics. STAR protocols 3:101562 (https://doi.org/10.1016/j.xpro.2022.101562)
19. Wang^ B., Lane^, B. J., Kapsalis, C., Ault, J. R., Sobott, F., El Mkami, H., Calabrese, A. N., Kalli, A. C., Pliotas*, C. (2022) Pocket delipidation induced by membrane tension or modification leads to a structurally analogous mechanosensitive channel state. Structure 30: 1-15 (https://doi.org/10.1016/j.str.2021.12.004)
2020
18. Hartley^ A. M., Ma^, Y., Lane^ B. J., Wang^ B., Pliotas*, C. (2020) Using pulsed EPR in the structural analysis of integral membrane proteins. Electron Paramag Reson, The Royal Society of Chemistry 27:74 -108.
17. Kapsalis, C., Ma, Y., Bela*, B. E., Pliotas*, C. (2020) In-Lipid Structure of Pressure-Sensitive Domains Hints Mechanosensitive Channel Functional Diversity. Biophys J 119(2):448-459 (doi: 10.1016/j.bpj.2020.06.012)
16. Koo, C. Z. et al., (2020) The tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex. J Biol Chem 295(36):12822-12839 (doi: 10.1074/jbc.RA120.012601)
2019
15. Kapsalis, C., Wang, B., El Mkami, H., Pitt, S. J., Schnell, J. R., Smith, T.K., Lippiat, J.D., Bode, B.E., Pliotas*, C. (2019) Allosteric activation of an ion channel triggered by modification of mechanosensitive nanopockets. Nat Commun 10:4619. (doi: 10.1038_s41467-019-12591-x)
14. Michou, M, Kapsalis C, Pliotas C., Skretas* G. (2019) Optimization of Recombinant Membrane Protein Production in the Engineered Escherichia coli Strains SuptoxD and SuptoxR. ACS Synth Biol 8(7):1631-1641. (doi: 10.1021/acssynbio.9b00120)
2017
13. Ackermann, K., Pliotas, C., Valera, S., Naismith, J.H., Bode*, B.E. (2017) Sparse labeling PELDOR spectroscopy on multimeric mechanosensitive membrane channels. Biophys J 113 (9): 1968-1978.
12. Pliotas, C et al., (2017) Adenosine Monophosphate Binding Stabilizes the KTN Domain of the Shewanella denitrificans Kef Potassium Efflux System. Biochemistry (doi: 10.1021/acs.biochem.7b00300)
11. Pliotas*, C. (2017) Ion Channel Conformation and Oligomerization Assessment by Site-Directed Spin Labeling and Pulsed-EPR. Methods Enzymol 594:203-242 (doi: 10.1016/bs.mie.2017.05.013)
10. Asadi, J., Ferguson, S., Raja, H., Hacker, C., Marius, P., Ward, R., Pliotas, C., Naismith, J.H., Lucocq*, J.M. (2017) Enhanced imaging of lipid rich nanoparticles embedded in methylcellulose films for transmission electron microscopy using mixtures of heavy metals. Micron (doi: 10.1016/j.micron.2017.03.019)
9. Pliotas*, C., Naismith*, J. H. (2017) Spectator no more, the role of the membrane in regulating ion channel function. Curr Opin Struct Biol (doi: 10.1016/j.sbi.2016.10.017)
2016
8. Hacker, C., Asadi, J., Pliotas, C., Ferguson, S., Sherry, L., Marius, P., Tello, J., Jackson, D., Naismith, J.H., Lucocq*, J.M. (2016) Nanoparticle suspensions enclosed in methyl cellulose: a new approach for quantifying nanoparticles in transmission electron microscopy. Sci Rep (doi: 10.1038/srep25275)
7. Valera, S., Ackermann, K., Pliotas, C., Huang, H., Naismith, J. H., Bode*, B. E. (2016) Accurate extraction of nanometer distances in multimers by pulse EPR. Chemistry (doi: 10.1002/chem.201505143)
2015
6. Pliotas, C., Dahl, A. C. E., Rasmussen, T., Mahendran, K. R., Smith, T. K., Marius, P., Gault, J., Banda, T., Rasmussen, A., Miller, S., Robinson, C. V., Bayley, H., Sansom, M. S. P., Booth, I. R., Naismith*, J. H. (2015) The role of lipids in mechanosensation. Nat Struct Mol Biol (doi: 10.1038/nsmb.3120)
2014
5. Ward^, R., Pliotas^, C., Branigan^, E., Hacker, C., Rasmussen, A., Hagelueken, G., Booth, I. R., Miller, S., Lucocq, J., Naismith*, J. H., Schiemann*, O. (2014) Probing the structure of the mechanosensitive channel of small conductance in lipid bilayers with pulsed electron-electron double resonance. Biophys J 106,834-842 (^: joint first author)
4. Healy, J., Ekkerman, S., Pliotas, C., Richard, M., Bartlett, W., Grayer, S.C., Morris, G.M., Miller, S., Booth, I.R., Conway, S.J., Rasmussen, T. (2014) Understanding the structural requirements for activators of the Kef bacterial potassium efflux system. Biochemistry 53, 1982-1992
2013
3. Branigan^, E., Pliotas^, C., Hagelueken, G., Naismith*, J.H. (2013) Quantification of free cysteines in membrane and soluble proteins using a fluorescent dye and thermal unfolding. Nat Protoc 8, 2090-2097 (^: joint first author)
2012
2. Pliotas, C., Ward, R., Branigan, E., Rasmussen, A., Hagelueken, G., Huang, H., Black, S.S., Booth*, I.R., Schiemann*, O., Naismith*, J.H. (2012) Conformational state of the MscS mechanosensitive channel in solution revealed by pulsed electron-electron double resonance (PELDOR) spectroscopy. Proc Natl Acad Sci U S A 109, E2675-2682
2010
1. Roosild*, T. P., Castronovo, S., Healy, J., Miller, S., Pliotas, C., Rasmussen, T., Bartlett, W., Conway, S.J., Booth*, I.R. (2010) Mechanism of ligand-gated potassium efflux in bacterial pathogens. Proc Natl Acad Sci U S A107, 19784-19789